FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_32.35200000038e7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_32.35200000038e7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716239
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10810.1509272742757655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC504.6002642E-520.999568
CAGCGTG400.006508943618.7496079
ACCGCAC400.006508943618.7496073
TAAGTCC550.00210089716.3632954
GTTATGC550.00210089716.3632953
CTAATAC1301.12449925E-814.9996853
GTATTAG1554.965841E-1014.5168381
ATTAGGC1054.265268E-614.2854163
TTAGGCT750.001017448713.9997064
CATGCAC650.006383837713.8458633
GAGTCTT1004.266946E-513.4997179
AGGACTA1004.266946E-513.4997175
CCTAATA1251.772778E-613.2006452
ACAGCGT800.001655607813.1247258
GTGTTAA1404.4957233E-712.8577721
CTAATAG2001.2732926E-1012.7497333
CCCTTAG1302.7527585E-612.6929271
AGGCTGT850.00261101412.3526836
TAATAGT2204.7293724E-1112.272474
TAATACT2451.8189894E-1212.2446414
Produced by FastQC (version 0.11.2)