FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_31.35200000038e39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_31.35200000038e39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664197
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31410.1887396744495994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGG750.001018166414.00021122
ACGCCCG650.00638953913.8455311
CTGCGCA1151.094154E-513.0428919
GCCCGCT954.1896998E-412.6313913
TATTAGG4700.012.4462482
GTATTAG4950.012.4240561
CTCTTAG2204.9112714E-1112.2725441
CGGGGGC900.004007922511.66684217
GTATTAA4500.011.66649151
CTAATAC5100.011.4700723
TAGTCAG2659.094947E-1211.3202459
TACACTG3100.011.12853055
CTAGGTT1355.653342E-511.110614
CGGTCGG950.00599918711.05246613
CACGCCC950.006000528611.05213410
ATTAGGT4350.010.6891743
GACTGTG1551.9563158E-510.6446827
TGGACTG1704.6818495E-610.5877595
GAGAGTC2556.4028427E-1010.5877587
TATACGG3550.010.5629045
Produced by FastQC (version 0.11.2)