FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_30.35200000038de7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_30.35200000038de7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825840
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34920.1912544363142444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA600.003756119414.99935710
TAGGTAG2550.014.1170415
CTCGGAG954.1910342E-412.6310367
GCTCGGA954.1920025E-412.630696
GTATTAG5100.012.3541021
CCTGGGG850.002614119312.3524113
CCTATAC2950.012.2028673
GGTAGTC2750.011.9994867
GTATAGA4550.011.8692471
TAGGGGG900.00400991311.6661665
TAGGGGT950.006000813611.0521564
TACACAG4000.010.8745335
ACAGTGC2905.456968E-1210.8616038
TGGACTG1807.8839184E-710.832875
CTAGGGT1253.4247802E-410.7995374
TTAGGTA4600.010.7604084
TAGGACC1551.956556E-510.6447054
CCTCTCG1000.00878319610.4995499
AGACTGT3151.8189894E-1210.4754536
TAGTCAG2752.1645974E-1010.3631929
Produced by FastQC (version 0.11.2)