FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_29.35200000038d97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_29.35200000038d97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737369
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29120.16760975935451825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGG600.003754327315.00037415
CGCCGCG952.635331E-514.2112924
ACGACCG800.00165776813.1241945
TAGGTAG1951.1677912E-912.3069365
GCCGCGT1101.042968E-412.27338625
ACCGCTC900.004010332711.6659518
CCTAGCG1355.65296E-511.11074830
CCGTCCA950.00600143811.0519539
CGATCAC1501.3595296E-511.0005924
GTCTAGT2207.750714E-910.9090491
GTCTTAC1704.6799305E-610.5881941
TATACTG6750.010.4438045
CGCGTCG1304.9146014E-410.38517319
ACCTAGC1451.16724055E-410.34478829
ACTATGC4350.010.34419258
GGTGCGA1602.7744603E-510.31305428
GTGCGAC1602.7762215E-510.3124629
TATACCG3500.010.2850835
GTTCTAA3950.010.2531251
GTATTAG4300.010.11624051
Produced by FastQC (version 0.11.2)