FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_28.35200000038d54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_28.35200000038d54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476043
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28640.194032287677256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT550.00210319916.36226811
ACCCCCC653.4396324E-416.1524968
CTAATAC4650.012.580023
CCGTGCA1201.6954793E-512.4989559
TAGACAG1702.8316208E-812.351915
GGTCGGG900.00400949311.66608815
TAGTCAG1501.3612149E-510.9990819
GGCGTAT1408.172474E-510.7148431
ATGGGAC1408.180911E-510.7137543
CTAGACT1704.680942E-610.5877094
GTGTTAT3700.010.5410881
TACACAG2853.8198777E-1110.5254365
CGATTCG1000.008782295510.49947814
GACTCTG1150.002076793510.433917
TAGACTG1304.9198855E-410.3837485
GTCTTAT3253.6379788E-1210.1543741
GTATTAG3850.010.1303971
TACTGCG2251.2554301E-79.9991647
ACTATGC3501.8189894E-129.8563198
GTGTATA5200.09.8082021
Produced by FastQC (version 0.11.2)