FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_27.35200000038d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_27.35200000038d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850696
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36470.19706099759225718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC750.001018260814.00017726
ACGGTGT3450.012.6078338
CACCGCG850.002613584312.35276223
TAGTCAG2204.9112714E-1112.2718879
GTATTAG4600.011.4141131
CCATGCG1602.2650438E-611.249239
GTGCGAC2155.4023985E-911.163836529
TACGGTG3900.011.1530837
CCTATCC1501.3615047E-510.9992483
ATACGGT4250.010.9404286
TATACGG4800.010.9367515
CGGTGTT3500.010.7135529
GACGGCC1408.185053E-510.7135527
TGCGACG2402.6502676E-910.62599530
TACAGGC3400.010.587517
CTAATAC4850.010.5147453
CCGTCCT1150.0020769610.4340699
GTCTAAG2453.7216523E-910.4091391
GTATATA16750.010.3890321
TAGGTAG3051.2732926E-1110.3271625
Produced by FastQC (version 0.11.2)