FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_26.35200000038cdb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_26.35200000038cdb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258505
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25870.20556136050313667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTC500.001109997218.00073814
CGGGTCC1002.5728732E-615.00061525
CCGTGCA600.003756135714.9988299
CTAATAC4800.014.3738783
CTAGCCT1056.755697E-512.8561384
ACTATGC4450.012.8079888
TCGGGTC1551.1320844E-712.58116224
CTATGCA3200.011.7178349
CGCATGT900.004006215411.66714614
CTTTGCG900.00400978511.6657559
CCTAATA5150.011.6495762
TAGGTAG1951.5326805E-811.537565
TACGGTG1951.5326805E-811.537567
CAGTCCT1303.847234E-511.537569
TAGACTG1059.71827E-411.4276785
TTCGGCG1202.3426866E-411.2509130
TAGCTAG1202.3461442E-411.2491225
GTATTAA4000.011.2491211
TTCGGGT1755.470356E-711.14331423
GGTCCCA1355.6458553E-511.11156727
Produced by FastQC (version 0.11.2)