FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_25.35200000038c98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_25.35200000038c98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601462
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17310.2877987304268599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTT254.224578E-429.9992513
TAGGTAG701.4435736E-619.2852345
CCCCGAC400.006507908518.7495316
GCCTCGG400.006507908518.7495320
CGACCGT500.001109572717.9995519
TATACAG2050.015.365475
GCCGCGT600.003749697215.00087425
TCCGCCA706.0320715E-414.99962620
GCAACTA706.0320715E-414.99962613
TATAGGC600.00375177214.9996265
TCTATAC1851.8189894E-1214.5942313
TATTAGG1658.185452E-1114.5463012
ATTAGGT1351.8473656E-814.4440843
TTCTAGG1004.2615502E-513.5007862
CTATTAG1454.712092E-813.4501771
GAACGGT902.649214E-413.3336
TCTAGGG800.001655120513.1246723
CATATAC2102.1827873E-1112.8568223
GTCTAAT1302.7478254E-612.6941011
GTGGACT954.1831454E-412.6312644
Produced by FastQC (version 0.11.2)