FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_24.35200000038c55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_24.35200000038c55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655398
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37500.5721714134007122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT500.001110137617.99853513
CTAGGAC500.001110137617.9985353
CTAATAC1356.184564E-1116.665313
GACGCTC550.002099347316.36480130
TTAGCCT550.00210148916.3623054
TAGGACT550.00210148916.3623054
CCTAGCG653.4319345E-416.15499730
TTACTCT1401.07320375E-1016.0701224
TAGAACT859.198298E-615.8810614
ATCACAC1256.686605E-915.6011129
AAGCGGG600.003749851415.00106830
TTATACT1709.094947E-1214.9987794
GTATAGA1554.965841E-1014.518271
CTCCGTC1351.8460923E-814.44547416
CTATGCA1701.3096724E-1014.1164989
CACCTAG750.001016577814.00099828
CTACGGT750.001017751313.9988624
CAGCGCA650.006385629513.8450279
ACCCTGC1106.904351E-613.6352548
TAGCGCG1004.2621705E-513.50096127
Produced by FastQC (version 0.11.2)