FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_23.35200000038c2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_23.35200000038c2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646944
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29650.18003040783414614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCG400.00651471218.74848210
TAGTCAG2800.017.141479
GGTAGTC3200.015.4679667
TTAGGTA4400.014.3174584
GTATTAG6200.013.5489361
CGGCTGG902.652128E-413.33387315
ATTAGGT4800.013.1243363
TATTAGG5600.013.1243362
GCGCAGG800.001657569813.1243356
TAGGTAG3850.012.8564925
AGGCCCG954.19199E-412.63055710
CACCTAG1551.1329212E-712.58115528
CAGCGCA1551.5458463E-611.6119639
CTAGGAC1303.847835E-511.5378773
CTAATAC4850.011.442723
CTAGCCT1459.342355E-611.3787354
CCTAGCG1355.6465928E-511.11189830
CGCGTTC1355.6483386E-511.11156126
GCTGGAC950.0059953111.05341517
GACATAG4500.011.0007830
Produced by FastQC (version 0.11.2)