FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_22.35200000038be9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_22.35200000038be9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777259
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16070.20675218942463194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC559.575673E-519.0905428
GTATTAG1800.017.5007881
CTAGACT755.6180295E-515.9996934
TAGTCAG1301.12504495E-814.9997119
GTAGACT600.003752946214.999714
TAGGTAG1453.0322553E-914.4824795
GGTAGTC1251.131375E-714.3997227
CCATGCG902.650649E-413.3330769
CTAGTAC1352.8931026E-713.3330763
ACCGTCG902.650649E-413.3330768
ACTGAGC800.001655808513.1247478
TATTAGG2650.013.0186172
TAGACTG1201.6919228E-512.4997595
CCTGTAC850.002611326512.3527033
TCTAGAC1354.20373E-612.2219863
ATTAGGT2351.2732926E-1112.1274253
GGCTCCT1006.4458774E-411.99976817
GTCAGAT1652.5506233E-711.81795411
GCTCTGA1151.5764784E-411.7396591
TTGGACC1151.577423E-411.7389034
Produced by FastQC (version 0.11.2)