FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_21.35200000038ba6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_21.35200000038ba6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130687
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880.2968925753900541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCTT200.0051307730.0000024
TACCCTT402.4513088E-422.5000025
GCATAAA350.002992078421.428571
CATAGGA350.002992078421.428574
ACCATCC400.006480370618.7500028
CTATACT400.006480370618.7500024
GTATAGA400.006480370618.7500021
TATAGAT601.8497588E-417.52
ATCATAC600.003730255215.03
TATAAAA800.001642770113.1250012
Produced by FastQC (version 0.11.2)