FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_20.35200000038b62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_20.35200000038b62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353229
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9440.2672487253311591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG200.005143319729.9990111
ACGCGCG254.2212833E-429.9990128
TAGGTAG603.274654E-722.4992565
CACTAAG350.003002654321.42786624
GTATTAG751.2597411E-719.999341
ACAGGCA455.457485E-419.999348
GGTAGTC701.4402394E-619.2850787
TCTAGTA559.557862E-519.0902792
AACAGTC559.557862E-519.0902797
TTAGGTA802.485358E-718.749384
ACCGAGC400.00650301818.749388
TATTATA1201.0913936E-1118.749382
TAGGGAG400.00650301818.749385
CAGATCC651.6411208E-518.4609289
ACCTAAT1007.21775E-917.9994051
CCTAATA854.6968307E-717.6464772
CCAGGAT601.8615348E-417.499423
GTCCATT601.8615348E-417.4994211
ATACTAT1303.45608E-1117.3071216
CTAATAC1051.3196768E-817.1422923
Produced by FastQC (version 0.11.2)