FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_19.35200000038b2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_19.35200000038b2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747769
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32960.18858327387658208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2900.017.067815
TAGTCAG2800.015.5346629
TACACCG2350.014.0416025
GTATTAG5700.013.9480211
TTAGGTA3950.013.6699614
CAGCGTG2200.013.635449
GGTAGTC3450.013.4773497
CTAGCTA1151.0944848E-513.0425944
AGACTGT2650.012.4519876
TGTTAGG1702.831257E-812.3524582
GTTAACC1354.213147E-612.2217453
ACAGTCC1354.2144948E-612.2213958
GACCTAC1501.0404983E-611.99953111
ACAGCGT3550.011.8301868
CTCTATA4850.011.7521172
ATTAGGT4600.011.738673
TAGACTG2052.566594E-911.7065255
CTAATAC6600.011.5904573
TACAGCG2850.011.5781647
TAATACT9150.011.3107094
Produced by FastQC (version 0.11.2)