FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_18.35200000038aea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_18.35200000038aea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078795
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41920.20165528587474954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3600.014.9989065
CGATCAC2000.014.25067524
CGCGCGC750.00101843813.9999914
ACTATGC6650.013.0817538
GTATTAG5850.013.0765971
CCCGCCG1302.7599672E-612.69290923
ACCTAAT6100.012.5406721
ATCACAC3950.012.53314329
TATTAGG5300.012.4519212
GCGCGCT850.00261350412.35293115
CCGTGCG850.002614918912.3520399
TAGTCAG3100.012.0958919
CCGCACG1151.5806491E-411.7382749
GGCGCGC900.004008972611.666656519
TTAGGTA4800.011.5616574
CTAGGCA2751.8189894E-1211.4537110
CCTAATA6700.011.4170782
TACTATG6700.011.4170787
GGTAGTC3700.011.3505237
GGACAGC2002.2308086E-811.249186
Produced by FastQC (version 0.11.2)