FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_17.35200000038ab3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_17.35200000038ab3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568154
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26420.168478350978284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC3000.017.4997047
GCCGCGT1405.456968E-1217.14420525
TAGGTAG2900.016.5514435
CGCCGCG1401.07320375E-1016.07166724
TAGTCAG3100.015.9669629
CGGGTCC851.6329522E-414.11875825
TCGCCGC1853.45608E-1113.78398923
TATTAGG5250.013.4283432
GTATTAG5400.013.3335311
CCTAATA5250.012.5712152
GTCAGAT4350.012.41318511
CTAATAC5500.012.2725193
TAGGACT1101.0435316E-412.2725184
AGTCAGA4550.012.19720610
ACTATGC5550.012.1615678
ATCACAC3950.012.15324229
TAATACT7850.011.6558514
TTAGGTA4650.011.6127054
AGGTAGT4700.011.4891666
ACCGTGC1059.7182166E-411.4280138
Produced by FastQC (version 0.11.2)