FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_16.35200000038a70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_16.35200000038a70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739743
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34080.1958910022917178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTC400.00651346818.7491748
GCCGCGT1001.4228135E-716.5011725
GTATTAG5400.015.5557641
GGTAGTC2950.014.7451137
CGCCGCG1054.2734737E-614.28549624
CAGTCCG750.00101865413.9993849
TATTAGG5050.013.9597812
GGACCGC650.006389651413.8455446
TAGGTAG3450.012.6081395
TAGTCAG3450.012.1733769
AGACCGC1252.576583E-511.9994726
TTAGGTA4350.011.7236214
AGTCCGG900.00400981911.66615310
GCGCACT1059.71876E-411.42806711
CCTGGAC1059.71876E-411.4280673
TCTGGGG1059.71876E-411.4280673
GTAGTCA3950.011.3919038
CTAGGAC1755.480979E-711.1423653
TCGCCGC1355.6519726E-511.11094123
AAGACCG1501.3610068E-510.99951655
Produced by FastQC (version 0.11.2)