FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_15.35200000038a3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_15.35200000038a3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762238
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34530.19594402118215587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2950.015.761495
CTAGACT1802.1827873E-1114.1655674
CCTAGCG1106.909804E-613.6372430
CAGCGTG1302.7631559E-612.6913239
GTATTAG5250.012.2868531
CGATCAC1406.3167263E-611.78613824
CCCTTAG2402.2191671E-1011.2510421
ACAGTCC1355.6556513E-511.11024958
CCTAATA6100.011.0650292
GGGTTAT2053.2006938E-810.9766271
ACTATGC4800.010.9366518
GTACCCG1100.001434268410.9082459
ACCGTGC1100.001434268410.9082458
GTCCTAA1807.8695666E-710.8343371
TCTATAC5350.010.6536823
GTCTAGA1704.674652E-610.5892161
CTATGCA3600.010.4158589
TGCGACG1304.914197E-410.38528330
GCAGGCC1451.1681336E-410.3440268
TTAGGAC1756.618877E-610.2852093
Produced by FastQC (version 0.11.2)