FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_14.352000000389f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_14.352000000389f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607427
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29900.18601155760106058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACGC800.001657761713.1241028
TGTCCGA954.1914507E-412.63071510
CGCGTCG850.00261133712.35401719
GTATTAG3650.012.3290741
GCGTGTG900.004010320611.6658699
GTCTTAG1859.158248E-811.3516331
GTCCTAC950.00599725611.0529061
CTCCTGG1253.422152E-410.8002691
ACAGGGC1851.1218071E-610.5398198
TATACTG6000.010.4989555
CGTCGCC1304.91266E-410.3855221
TTAGACC1602.7787974E-510.3114744
GTCTATG2957.2759576E-1110.16974451
TCCGCGT1501.6450374E-49.99931610
CCATGCC1200.0029566289.9993169
TAATACT7600.09.8674384
TATACCG3203.274181E-119.8427715
ACCGTTC1851.2818369E-59.7290648
TAGGACA1705.4107437E-59.7049164
CTAATAC4200.09.6418973
Produced by FastQC (version 0.11.2)