FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_13.352000000389b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_13.352000000389b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919008
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36040.18780536610582133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5050.013.9592321
ACAGTGC2200.013.6352268
TAGTCAG3000.012.4989579
TAGGTAG3650.011.9165055
GGTAGTC3200.011.71777257
CTTACAC2850.011.5776813
GCAGTGC1355.6565936E-511.1101858
GTCTTAA2556.4028427E-1010.5873521
TGTATCG2156.463779E-810.463975
GACATAG3450.010.4363630
TTAGGTA5100.010.2929924
AACGGGC1356.949995E-410.00151230
GTCCTAA1653.899343E-59.99916651
GGACTGC1356.9649884E-49.99916656
CTGCGCA1356.9649884E-49.99916659
CCGTTCC1356.9649884E-49.99916659
ACAGGTC1952.2048753E-69.9991668
ATTAGGT4950.09.9989063
CTAATAC5250.09.9989053
TATACTG6050.09.916275
Produced by FastQC (version 0.11.2)