FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_96.35100000039e53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_96.35100000039e53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707010
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT42580.6022545649990807No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC31260.44214367547842326No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC30590.4326671475650981No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC25420.3595422978458579No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG23890.3379018684318468No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC23320.3298397476697642No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT22960.324747881925291No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG22260.31484703186659313No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT20160.28514448169049944No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC19250.27227337661419215No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT17100.2418636228624772No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG16150.2284267549256729No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG15690.22192048202995715No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG15620.22093039702408734No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG15080.21329259840737755No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG14830.20975658052927115No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT14030.1984413233193307No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13460.19037920255724813No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC13100.18528733681277493No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT12880.1821756410800413No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC12830.18146843750442002No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT12810.1811855560741715No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT12620.17849818248681065No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC12230.17298199459696467No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA12050.17043606172472808No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT11330.16025233023578167No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT11200.15841360093916634No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC11090.15685775307279953No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC10820.15303885376444462No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA10100.14285512227549824No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA9960.14087495226375862No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG9800.13861190082177055No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG9710.13733893438565226No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC9460.13380291650754586No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT9460.13380291650754586No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT9410.1330957129319246No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT9340.1321056279260548No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG9280.13125698363530927No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9110.1288524914781969No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8840.125033592169842No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8610.12178045572198413No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8520.12050748928586583No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8460.11965884499512029No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA8330.11782011569850498No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC8250.11668858997751093No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC8240.11654714926238668No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8140.11513274211114412No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7970.11272824995403176No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA7960.1125868092389075No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7960.1125868092389075No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG7950.11244536852378324No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7940.112303927808659No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7860.11117240208766495No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC7830.11074807994229219No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7670.1084850285003041No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7550.10678773991881303No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG7490.1059390956280675No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA7450.10537333276757048No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA7450.10537333276757048No Hit
GCTAACTTTTCAAGGCCGAGAGCTGATGACGAGTGT7350.10395892561632793No Hit
GAGTTAGCCGGTGCTTATTCTGTCGGTAACGTCAAA7300.10325172204070665No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7290.10311028132558239No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC7270.10282739989533388No Hit
CCCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTG7220.1021201963197126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG300.001223359825.0095521
ATAGCTA300.001226896824.9971717
CCGTATG7700.022.9844118
ACCGTAT7700.022.9844117
CCTAGAT402.4669143E-422.49904629
ATTGAAA402.4680956E-422.49745424
CGTATGC7950.022.450299
CCACCGT7850.021.982925
GTCTCTT908.367351E-1121.66574730
TATGCGC4850.021.64703411
ACTCGGT1250.021.6082523
GTATGCG8350.021.55444710
CCATGAT2900.020.6975614
TCCACCG8200.020.678634
CACCGTA8400.020.543566
ATCCACC8350.020.1274493
ACCTAGA455.465264E-419.99915128
TCTGACT751.265289E-719.99773815
TATGCGT3700.019.86261611
CATCCAC8600.019.5423492
Produced by FastQC (version 0.11.2)