FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_95.35100000039e1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_95.35100000039e1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972543
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT80330.8259789027323213No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC50730.5216221802018008No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC40090.4122182772381273No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG34800.35782479540750384No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT28500.2930461686526971No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG27510.28286667016265604No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC27430.2820440844260871No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG27060.27823962539445557No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT26740.2749492824481797No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG26590.27340693419211287No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC26590.27340693419211287No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC24970.2567495730265911No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT24260.24944912461454144No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG24230.2491406549633281No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG23590.24255996907077632No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT22360.2299127133710283No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC21250.2184993362761338No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC20540.21119888786408414No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG20300.20873113065437723No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT19240.19783186964483832No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA19010.1954669356522025No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC19000.19536411243513138No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC18440.18960601227914858No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT18350.18868060332550848No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT16890.1736684136331247No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG16260.16719055095764404No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC15890.16338609192601253No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT15680.16122680436751896No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT15160.15587999707982064No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG15110.15536588099446502No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT14850.15269247735061586No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC14770.15186989161404688No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT14240.14642026110927742No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG14170.14570049858977957No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT14000.1439525038995705No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA13770.14158756990693472No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA13540.1392226359142989No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC13490.1387085198289433No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG13440.13819440374358768No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA13110.13480123758024068No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC13100.13469841436316954No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA13030.1339786518436717No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA13000.13367018219245833No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT12880.13243630358760486No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT12860.1322306571534626No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA12750.13109960176568028No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG12720.1307911321144669No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT12610.12966007672668456No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC12350.1269866730828354No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA11960.12297656761706165No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG11790.1212285729268526No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA11740.12071445684149698No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC11730.12061163362442587No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC11660.119891871104928No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA11560.1188636389342168No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT11180.11495635668551415No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC11060.11372247808066069No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT10960.11269424590994949No Hit
CGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACC10830.11135754408802491No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT10790.11094625121974042No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA10730.11032931191731368No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG10630.10930107974660246No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG10390.10683332253689554No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC10260.10549662071497096No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC10220.10508532784668646No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC10200.10487968141254422No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC10190.10477685819547311No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT10140.10426274211011749No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC10080.10364580280769076No Hit
CTGTATTACCGCGGCTGCTGGCACAGAGTTAGCCGG10020.10302886350526404No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG10020.10302886350526404No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC10000.1028232170711218No Hit
CCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTAT9840.10117804559798384No Hit
GCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAA9830.10107522238091272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACT200.00514083630.0072024
GGTCCGA601.1881639E-825.0047151
GCCTCTG1450.024.82333212
GTAGGAT950.023.6898963
GTCCGAA603.2798926E-722.5042442
GTCTCTT603.2831485E-722.5019330
ACTCGGT2950.022.3782523
CCTCTGA1550.022.25653813
TAGGATA957.2759576E-1222.1105694
CTCGGTC2950.021.8696544
CTCTGAC1600.021.56102214
TACCTAG350.00300433121.43040827
TCTGACT1700.021.17501815
AGAGGCA951.72804E-1020.52807630
GCCATAC14550.020.3131081
CGTCTTT1700.020.2979451
TACCCTT14350.020.1800965
CATACCC14450.020.1432423
CCATACC14650.019.9696352
GAGCCAT5500.019.9128461
Produced by FastQC (version 0.11.2)