FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_94.35100000039dda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_94.35100000039dda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691651
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT53930.7797285046938413No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC24840.3591406648728911No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC21030.30405507980180757No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG20600.2978380715129451No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG20050.28988608416672573No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT15670.22655934857319662No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG15380.22236648251791727No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT15210.2199085955199949No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC15030.21730612693395945No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG14630.21152286340943627No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG14480.20935413958774007No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT14310.20689625258981773No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG14060.2032817128869907No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC14040.20299254971076455No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA13540.19576347030511054No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT13410.19388390965964047No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC12860.18593192231342107No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT12080.17465455844060082No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT11980.17320874255947002No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC11220.16222054186287593No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT10520.15209983069496033No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG10450.15108775957816875No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC10120.14631656717043712No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC9760.14111162999836624No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG9690.1400995588815747No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC9650.13952123252912235No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA9600.13879832458855695No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC9590.13865374300044386No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT9390.13576211123818227No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG9300.13446087694516454No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9130.1320029899472422No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8960.1295451029493198No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8690.12564140007026667No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG8640.12491849212970126No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA8410.1215931156031004No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT8170.11812315748838649No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT8080.11682192319536877No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8080.11682192319536877No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT8030.11609901525480336No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT7830.11320738349254175No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT7730.11176156761141096No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC7700.11132782284707173No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC7690.11118324125895863No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG7680.11103865967084556No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7660.1107494964946194No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT7550.10915909902537552No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT7430.10742411996801854No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7390.10684579361556625No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC7350.10626746726311391No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7230.10453248820575696No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7140.10323125391273923No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7130.10308667232462615No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC7050.1019300196197215No Hit
CTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG7010.10135169326726919No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC6950.1004842037385907No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA6940.10033962215047762No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG6930.10019504056236456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG303.4834047E-530.006873
TCATATA303.4834047E-530.006872
TATAGAA359.954341E-525.7201735
GGAACTC300.001224424925.0057243
GTCATAT452.0343587E-523.3403641
ACGAGAC804.5110937E-1022.49864622
CTTATAC908.367351E-1121.6731971
CCTCTGA908.367351E-1121.66536113
ACCCGTG657.0766873E-720.7739893
ACCGTAT4900.020.5089687
GGACAAC2300.020.21617311
TATGCGC3500.020.14164411
CCGTATG5100.019.9987968
CATACCC8500.019.7692323
GCCATAC8650.019.601281
CACCGTA5300.019.5327726
TACCCTT8450.019.3535445
CGACTTG701.4447014E-619.28455218
CCCCCCT559.5502E-519.096661
ACTCGGT1106.002665E-1119.0952823
Produced by FastQC (version 0.11.2)