FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_93.35100000039d97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_93.35100000039d97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77171
Sequences flagged as poor quality0
Sequence length36
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT3560.4613131875963769No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3110.4030011273664978No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2930.3796763032745461No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2340.3032227131953713No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCT1760.22806494667686047No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1670.21640253463088466No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG1450.1878944162962771No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT1410.1827111220536212No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG1410.1827111220536212No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG1390.18011947493229322No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1390.18011947493229322No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1350.1749361806896373No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1270.16456959220432546No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC1240.16068212152233352No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC1160.1503155330370217No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACG1150.1490197094763577No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1090.14124476811237383No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1070.13865312099104588No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT1030.13346982674838995No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT1020.13217400318772596No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT970.12569488538440607No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA970.12569488538440607No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC960.12439906182374208No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC960.12439906182374208No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC940.12180741470241412No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA930.12051159114175014No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT930.12051159114175014No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG930.12051159114175014No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG910.11791994402042218No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC880.11403247333843024No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT870.11273664977776625No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA850.1101450026564383No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG820.10625753197444636No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG820.10625753197444636No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC800.10366588485311841No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC800.10366588485311841No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT780.10107423773179043No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT780.10107423773179043No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA780.10107423773179043No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA780.10107423773179043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTTC300.001216210525.07
AACTTTT300.001216210525.06
TAACTTT300.001216210525.05
TATGCGC452.008852E-523.33333411
TCCTAGC350.002981097221.4285723
TTTTCAA350.002981097221.4285729
TTTCAAA350.002981097221.42857210
CTAACTT350.002981097221.4285724
GTATGCG656.925802E-720.7692310
CACCGTA656.925802E-720.769236
CATCCAC656.925802E-720.769232
ACCGTAT656.925802E-720.769237
CCGTATG656.925802E-720.769238
TTCAAAC455.403351E-420.00000211
GCTAACT455.403351E-420.0000023
TGGGGCG608.088715E-620.022
CCATACC852.1394953E-819.4117642
TACCCTT852.1394953E-819.4117645
CATACCC852.1394953E-819.4117643
CCACCGT701.4102079E-619.2857155
Produced by FastQC (version 0.11.2)