FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_92.35100000039d54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_92.35100000039d54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141110
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT8040.5697682658918574No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC4130.29267946991708593No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3790.26858479200623625No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3730.26433279002196863No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3450.2444901140953866No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC3340.23669477712422934No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2930.2076394302317341No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2860.2026787612500886No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2620.18567075331301822No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2560.1814187513287506No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2520.1785840833392389No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2150.1523634044362554No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG2100.14882006944936577No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG2090.1481114024519878No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2000.14173339947558644No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG1930.1367727304939409No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1930.1367727304939409No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1930.1367727304939409No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1920.13606406349656297No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1900.1346467295018071No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1860.13181206151229538No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1850.13110339451491745No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1840.1303947275175395No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1830.12968606052016157No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1820.12897739352278365No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1780.12614272553327194No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1770.12543405853589398No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1750.12401672454113813No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1710.1211820565516264No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1700.12047338955424845No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1620.114804053575225No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1610.11409538657784707No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1600.11338671958046914No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC1570.11126071858833535No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT1550.10984338459357948No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1540.10913471759620155No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1500.10630004960668982No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT1420.10063071362766637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCT352.8753748E-630.027
GGAGAAC408.125187E-626.25000218
CCAGCTA408.125187E-626.25000224
GAGAACC452.0242407E-523.33333219
CGAGGAG452.0242407E-523.33333215
AGCTATC452.0242407E-523.33333226
TCTAGGT350.002993252421.4285724
CTAGGTA350.002993252421.4285725
AGGAGAA504.5659952E-521.017
CGGAGTA504.5659952E-521.019
AGAAGCA801.0846634E-820.62500227
TTCAAAC801.0846634E-820.62500211
GCCATAC2100.020.01
CATACCC1950.020.03
TACCCTT2050.019.7560985
CCATACC2000.019.52
TATCAGC559.5037474E-519.0909082
GAGTACC559.5037474E-519.09090821
AACCAGC559.5037474E-519.09090822
CCCCGGA559.5037474E-519.09090816
Produced by FastQC (version 0.11.2)