FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_91.35100000039d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_91.35100000039d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124215
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT11230.9040776073743108No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC4660.375155979551584No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4320.3477840840478203No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3890.3131666867930604No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3800.30592118504206417No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3490.28096445678863263No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3380.27210884353741494No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT3000.24151672503320856No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2820.22702572153121603No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2810.22622066578110536No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC2600.20931449502878074No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA2240.18033248802479573No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG2230.179527432274685No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2150.17308698627379945No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC2140.17228193052368876No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2130.17147687477357806No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2000.16101115002213903No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1970.15859598277180695No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1930.15537575977136417No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1900.15296059252103208No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1850.1489353137704786No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1810.1457150907700358No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1770.14249486776959305No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1770.14249486776959305No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1720.13846958901903955No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1520.12236847401682566No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA1500.12075836251660428No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1490.11995330676649357No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT1480.11914825101638289No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1460.11753813951616149No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1440.1159280280159401No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1380.11109769351527593No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1350.10868252626494385No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1330.10707241476472246No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC1320.10626735901461176No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1310.10546230326450105No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA1300.10465724751439037No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA1290.10385219176427968No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC1260.1014370245139476No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1250.10063196876383688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCC200.005132161529.99718317
TGTATTC200.005132161529.99718320
ATTCAGT200.005132161529.99718323
GTCCCTC200.005132161529.99718316
CTCCATC254.2072008E-429.9971820
CCGTCCC300.001221071924.9976514
GAGAACC300.001221071924.9976519
CGTCCCT300.001221071924.9976515
TATGCGC501.6787817E-623.99774411
CCACCGT903.6379788E-1223.3405365
GCCATAC1750.023.159331
CATACCC1800.022.5069473
AGTCACA402.45192E-422.49788712
CCATACC1800.021.6733552
AATTTCG350.002992884721.4265618
AGGAGAA350.002992884721.4265617
GCTATCT350.002992884721.4265627
CCAGCTA350.002992884721.4265624
TACCTTA350.002992884721.4265626
CTACCTT350.002992884721.4265625
Produced by FastQC (version 0.11.2)