FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_90.35100000039cce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_90.35100000039cce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75368
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATG7240.9606198917312387No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCA3100.41131514701199445No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGCAT2490.330378940664473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCATG850.026.46707530
CTCTTAT359.8192E-525.7279451
TAGGCAT1051.8189894E-1221.42572829
AGCCCAC1301.8189894E-1218.45908717
CACGAGA1301.8189894E-1218.45908721
TCTCCGA1301.8189894E-1218.45908711
GCCCACG1251.8189894E-1117.99761218
CGAGACT1251.8189894E-1117.99761223
ACGAGAC1251.8189894E-1117.99761222
ACACATC1353.6379788E-1217.7872226
TCCGAGC1303.274181E-1117.30539513
CCCACGA1303.274181E-1117.30539519
ACTAGGC1303.274181E-1117.30539527
CCGAGCC1303.274181E-1117.30539514
TATACAC1750.017.1519643
TACACAT1405.456968E-1217.1519645
ATACACA1500.017.0090314
AGACTAG1355.820766E-1116.66445525
CTCCGAG1355.820766E-1116.66445512
GAGCCCA1355.820766E-1116.66445516
Produced by FastQC (version 0.11.2)