FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_89.35100000039c8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_89.35100000039c8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730829
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGCC1450.021.72245212
GACTCCT1400.020.35695830
CCCGGAG1550.020.32239317
CCCGGGG1350.019.9984526
GCCCGGC1650.019.9984511
CGGCCCG1600.019.6873214
AGCCCGG1350.018.88742410
GCCCGGG1550.018.3856725
CCGGCCC1750.017.99860413
GCCCGGA1850.017.83767516
CGGAGGG1750.017.142719
CCGGGGC1501.8189894E-1216.99868227
CCGGAGG2100.016.42842118
GGGCCCG1750.016.28445223
GGAGCCC1700.015.8811218
GAGCCCG1800.015.8321069
GGACTCC1900.015.7893329
AGGAGCC1850.015.4042117
GGAGGGC1950.015.38447720
AGGGCCC2050.014.63301222
Produced by FastQC (version 0.11.2)