FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_88.35100000039c48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_88.35100000039c48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051155
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG752.790559E-618.0034835
CCGAGCC1450.017.5845914
CCTGAGC1709.094947E-1215.00147530
ACTGCGC750.001018551613.99871358
CCACGAG2150.013.95220620
GTCTTAG1201.118955E-613.7533151
CTCCGAG1958.367351E-1113.07572112
TCCGAGC1855.0749804E-1012.97178113
GAGACTC1907.730705E-1012.63041824
ACGAGAC1907.730705E-1012.63041822
GCCCACG2153.274181E-1112.55698518
AGCCCAC2051.9281288E-1012.437880517
TAGAACT1951.1605152E-912.3100744
CGAGCCC1951.1659722E-912.306560515
GAGCCCA2351.2732926E-1112.12654416
TGGGGGG2001.7298589E-912.0023225
CGAGACT2001.7371349E-911.99889623
AGGGGGG1303.8351758E-511.5406945
GTATTAA3000.011.5027721
ACTCCTG2103.7307473E-911.42915227
Produced by FastQC (version 0.11.2)