FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_87.35100000039bf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_87.35100000039bf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254901
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT600.003755867714.99898510
GTTGGCC1006.453369E-411.9991879
CGAGCCC1551.544633E-611.61211715
TATTAGG2900.011.3821682
TTAGGCA1202.3389781E-411.2528254
TAGCCTG1355.6337638E-511.1139015
TGGGGGG1501.3557277E-511.0027625
TTAAGGT2351.8644641E-910.8537884
GACAGGC1807.878989E-710.83259926
GTATTAG2957.2759576E-1210.6806471
CCGAGCC1704.6801415E-610.58751814
TATACGG2701.5279511E-1010.5582065
CTTCGTG1000.00878224410.49928959
GGTGCGC1000.00878224410.49928958
CAGCGTG1150.00207614710.4340759
GTCTTAG1451.16364405E-410.3474251
CCCCTAT1602.7667542E-510.3150881
CCTATAC2208.990901E-810.2298413
CATATAC4350.010.0025113
TAGACAG2251.2485907E-710.0025115
Produced by FastQC (version 0.11.2)