FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_86.35100000039bb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_86.35100000039bb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127191
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1557.752533E-913.549315
CTGTGCC954.1896064E-412.6307569
TTGGGGG1201.6920023E-512.500854
TCTAGAT1354.2043666E-612.2230533
TGAACCG900.004004964611.667465
GTATAGA3100.011.6136941
ACTATGC3250.011.5377118
CTATGCA2555.4569682E-1111.1757449
ATACGGT2155.3951226E-911.163556
GTATTAG3450.010.8703051
TAGGTAG1408.1672035E-510.7150145
AGACTGT2251.0975782E-810.6673926
TAAGACT2251.0975782E-810.6673924
AGGGGGG1551.9523313E-510.6458865
TCACACA1704.673657E-610.58848630
CCTGGTC1000.00878113710.4993178
CCTAATA3151.8189894E-1210.4769042
TAGACTG1150.00207352310.4354925
ACAGTGC1150.002075819310.4341038
ACCTAAT2905.2750693E-1110.3455311
Produced by FastQC (version 0.11.2)