FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_85.35100000039b7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_85.35100000039b7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465104
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGA4760.10234270184732877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGA350.003001808221.43064529
ACTGTGT504.599258E-520.9975178
CCTACCC400.00649713418.753833
CTGTGCT400.00650924718.7477829
ACAGATC500.001109752817.9978718
TCTCCGA706.032698E-414.99822611
ACGAGAC851.6302947E-414.11749622
CCACGAG750.001017254813.99834420
CTATACT902.642057E-413.3360584
CGAGACT800.001653961113.12485923
GCCCACG800.00165530113.12344718
CTAGACA850.002604233812.3554654
CTAAGGC850.002606335812.35413628
CCCACGA850.00261054412.35148119
ACACATC1101.0383403E-412.2752346
TATACTG1354.183019E-612.2247195
CACGAGA900.00400449111.66528721
TAGACAG1459.275909E-611.3816365
GTCTTGA1202.3339751E-411.2522981
TAAGGCG950.005983202311.05370129
Produced by FastQC (version 0.11.2)