FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_84.35100000039b3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_84.35100000039b3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757427
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCG550.00210174216.36248416
TGGGGGG901.579216E-515.0009235
CCGAGCC706.036713E-414.99894414
CTAGACC650.00638079213.8470074
CCTAGAC800.001654731313.1258093
GTATTAG2153.274181E-1112.5589131
GTATTAC2001.7298589E-912.000741
CTATGCA1501.0383083E-611.9991559
AGACCGT1151.5761322E-411.7398556
TAGACAG900.00400308411.6673855
TAGGATT1059.6991006E-411.42927554
ACCGTCG1059.709834E-411.4277678
TCCGGGG1459.325857E-611.37850911
CTAATAC2301.3060344E-911.0876413
TAATACT3550.010.9865924
TGTAGAC1653.2633125E-610.9097633
CACGAGA1100.00143175710.90904221
ACGAGAC1100.00143175710.90904222
CCACGAG1100.00143175710.90904220
ATACCGT1951.8889295E-710.7698956
Produced by FastQC (version 0.11.2)