FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_83.35100000039af7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_83.35100000039af7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881984
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG805.1628194E-616.8775834
CAGTCGC550.002100564516.36428624
AGGGGGG1658.185452E-1114.5476825
TAGGTAG1251.1295742E-714.40220455
GCAGTCG650.006382815513.846704523
GTATTAG2400.013.7528851
TGGGGGG1251.7720304E-613.2020215
ACACCGA800.001653967313.1270096
GGTTAGA850.002607324612.3555331
TTGGGGG1252.5680118E-512.0018384
TCCGAGC1006.450344E-411.99911513
ACGGTGT1551.5430251E-611.6120478
TAATACT4900.011.3282644
TTCTATA3050.011.3132082
CCGAGCC1100.001432990510.90828714
CCTAATA3051.8189894E-1210.8213292
TCCTGGA1408.1555176E-510.7159262
GTATACC1551.949171E-510.6467913
ATAGGGG1000.00876441210.5016073
TACACCG1000.00876441210.5016075
Produced by FastQC (version 0.11.2)