FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_81.35100000039a70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_81.35100000039a70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380101
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCC303.480383E-530.0055264
GCTCGAG200.0051449629.99763511
TAGGACA452.0330752E-523.3376334
GGTAGTC553.8764247E-621.816467
TAGACAG701.4378329E-619.2892675
GGATTAG550.002095581516.366651
ACAGCCT550.0020992716.3623458
TAGTCAG550.0020992716.3623459
TAGGTAG653.4242438E-416.1568225
TAGACCG809.709588E-515.0027645
AGAGTGA600.003743176615.0027636
GGTTAAG600.003743176615.0027631
TCTATAC1002.5576246E-615.0027633
TAGTGTG706.0273043E-414.9988169
GCTTAAT851.6263613E-414.1202481
TGGGGGG750.001014331414.0025795
ATAGGAG902.6405166E-413.3357915
CTAGACA800.001650566913.12741854
AGACCGT800.001650566913.12741856
AGGTAGT954.16949E-412.6339066
Produced by FastQC (version 0.11.2)