FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_80.35100000039a3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_80.35100000039a3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260819
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG350.002997994721.4310361
TAAGGGA500.001106242818.0020714
CATGGAC550.00209417616.3655193
TGATACC600.00374069915.0017274
TAGAATG600.00374069915.0017275
GTCTAAA600.00374069915.0017271
TGGACAG809.696711E-515.0017265
TTGATAC706.009455E-415.0017253
CTATACT901.5705476E-515.0017254
CTCCGAG600.00374547614.9988512
GCCAAGA600.00374547614.9988513
TCAGTAC851.6242251E-414.1192723
TAATAGT750.001013361514.0016114
TAGCACT750.001013361514.0016114
CTATTAG750.001013361514.0016111
TATAAAA750.001013361514.0016112
GCCCACG650.006371849713.84509218
TGCCAAA650.006371849713.84509212
TCCGAGC650.006371849713.84509213
CCACGAG650.006371849713.84509220
Produced by FastQC (version 0.11.2)