FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_79.35100000039a06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_79.35100000039a06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721617
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACC400.006510807718.74879
CTCCGAG1151.1277734E-1018.25833912
GTGGCGT752.7951046E-617.99875330
CGAGCCC1051.3282261E-817.14048215
CCACGAG1102.3557732E-816.36250320
TCCGAGC1102.357774E-816.36136813
TAGAACC951.5016685E-615.7949484
GCCCACG1154.071262E-815.65108918
CACGAGA1154.071262E-815.65108921
CCGAGCC1154.074718E-815.65000414
CTAACAC809.715406E-515.0051993
TCCCGCC706.036171E-414.99896122
ACTCCCG706.036171E-414.99896120
ACAGCGT1002.5721347E-614.99896058
GTATTAG1950.014.620451
CTAATAC2300.014.3527983
TGGACTA750.00101410714.0058245
TCTCCGA1504.8894435E-913.9990311
ACGAGAC1301.8271749E-713.84519422
CCTGCCC650.00638594413.84519410
Produced by FastQC (version 0.11.2)