FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_78.351000000399c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_78.351000000399c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380016
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3600.016.6708935
TAGGGGG2100.015.718274
CGGGGGG650.006377976413.8496665
TGGGGGG3900.013.8496655
TAGGTAG2450.013.4728035
CTAGACT2700.013.3367154
CGCCGCG1406.3276893E-611.7847424
CCGAGCC1703.7715836E-711.4696414
GACCGTG1100.001434648810.908197
CTAGGGT1704.667905E-610.590924
TCTAGAC2556.384653E-1010.5906973
TAATACT11600.010.4767944
GTATTAG5500.010.3669181
GCCGCGT1901.5805472E-610.26274125
GGTAGTC2957.2759576E-1110.1686517
CCTAGAC2402.993329E-810.0023263
CCGTCCC1356.96625E-49.9991749
CGAGCCC1851.28287975E-59.72892615
ATACGGT3401.0913936E-119.70834356
TTAGGGG2054.1592357E-69.5144073
Produced by FastQC (version 0.11.2)