FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_77.3510000003998e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_77.3510000003998e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656260
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG1253.7107384E-1016.8030094
CTGAGCG750.001018758713.9991279
TGGGGGG2503.6379788E-1212.002155
AGGGGGG1901.0406438E-811.8442285
GTATTAG4600.011.7415881
CTAATAC4950.011.5172153
TAGAGTG2659.094947E-1211.32278255
GACGGAC1202.3453402E-411.24997726
TAATACT8100.011.1131024
TAGGTAG2453.1832315E-1011.0223835
GTCTTAG2207.723429E-910.9113751
ATAGGGG1807.8597077E-710.8352753
ACCTAAT4850.010.8270091
TCTATAC4300.010.4669913
TGTGCTG1451.16500625E-410.3466815
CCTAATA5700.010.2649972
ACTATGC4750.010.1046338
GACTCCT1653.894983E-59.99997930
CGTCGCT1501.6450057E-49.99937610
TACTGAG2557.197741E-99.9993757
Produced by FastQC (version 0.11.2)