FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_76.3510000003994b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_76.3510000003994b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477037
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCC300.001225377825.00043726
TGGGGGG504.591055E-521.002575
GCCACCG550.002098973616.36392227
CTTTCGC600.003749198315.00026225
GTGTAAG750.001014668114.00318051
GCAGCGT750.001014668114.00318051
GTTGTAA650.006369238313.84929851
AGTAACC650.006373664413.8478473
CTTAACA902.6488118E-413.332179
TACGGTG1056.739194E-512.856027
ATAGTGG1006.43017E-412.0014693
TTAGACA1252.5630208E-512.0014684
CAGCGTC900.003997553611.6680952
GCGTCAG900.003997553611.6680954
CAAGACA1059.682666E-411.4299694
TCAACAT1355.6241894E-511.112473
GTATTCT950.005977777811.0551431
GTATTGG1501.3512072E-511.00251
CCTTATA1501.3527791E-511.0013462
TGCTAGA1100.001428560210.9104252
Produced by FastQC (version 0.11.2)