FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_75.35100000039907.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_75.35100000039907.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3166646
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG400.006503804618.754575
TGGGGGG4350.015.5210255
TAGGGGG2550.014.7094674
AGGGGGG4250.014.1210895
CCCGAGC902.6562222E-413.3321628
ACCGACC1456.9157613E-712.4127028
TACCGCG900.004013792611.6650897
CTCCTAG1703.7510472E-711.4748341
CCGAGCC2155.4224074E-911.16216314
CTAGGAC950.00598966811.0553263
GTCTTAG2504.5110937E-1010.8039991
GCCCACG2156.468326E-810.46419718
ACAGCCC1304.923419E-410.3837028
TCTTATA7550.010.3339712
TAGCCTG1602.7706246E-510.3150145
CGAGCCC2053.783607E-710.24284115
TATAGGG3600.010.0027542
ACAGCGT1356.967942E-49.9991218
GTATAGG4550.09.8937711
TTAGGGG2003.03675E-69.7523773
Produced by FastQC (version 0.11.2)