FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_74.351000000398c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_74.351000000398c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483330
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1303.45608E-1117.30638918
CCGAGCC1051.33040885E-817.14156514
TGGGGGG2750.014.731135
CACGAGA1555.002221E-1014.51503621
TCCGAGC1251.1345037E-714.39891613
CAGCCGG650.006390388613.84511118
CCGTACT1404.5115848E-712.85660828
CCCACGA1804.6784407E-912.49905919
GTCTAGG850.002606432212.3570111
AGGGGGG2102.8740033E-1012.1460385
GTTCTAG1754.21569E-812.0039521
TTAGGGG1252.5688094E-512.0027383
TAGGGGG1901.03900675E-811.8452074
CGAGACC1551.5460264E-611.61163823
AGCCCAC2103.74348E-911.42771117
CGTACTA1602.2624008E-611.24991129
CTGTGTG1602.2633176E-611.2495329
CGAGCCC1602.2642344E-611.24915315
CCACGAG2155.4133125E-911.1619520
GAGCCCA1755.481106E-711.14201716
Produced by FastQC (version 0.11.2)