FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_73.3510000003989c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_73.3510000003989c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729183
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA755.6086545E-516.0025621
CTCTTAT1503.0377123E-1015.0024011
TAAGACA600.003748174815.00244
CTAAGCC650.00637633313.848373
TACTAGG650.00637633313.848372
TTCTATA902.6462757E-413.3354672
CTAGACT1251.770688E-613.2021134
ATAATAC1251.770688E-613.2021133
GTATAGA800.00165340313.12711
TCTTATA1753.0540832E-912.85920052
TGTAAGA1101.04033286E-412.2746922
ATATATA1501.0352451E-612.0019211
TCTAAAC1006.435323E-412.0019213
TAGGCTT1006.435323E-412.0019215
AACCGTT1006.4501807E-411.9986297
GTCCCAG1151.5745343E-411.7410091
TTATGAC900.003999896311.6685333
AGGGGGG900.003999896311.6685335
GTTATAG1303.8327264E-511.5403081
TAGACGG1059.690346E-411.4304015
Produced by FastQC (version 0.11.2)