FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_72.35100000039859.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_72.35100000039859.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length36
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGACGATGCAGCAAATGACGAATGGGAGAGTAT11.3513513513513513No Hit
CCGTACGCCTTGCCAAACGTGGTAGTGTGCATGTTT11.3513513513513513No Hit
GAATTACCTCATGCAATTAAGACTGGAATGATTGCT11.3513513513513513No Hit
CTCATAAAGATTCAAAAAAAAATATAAAGATTAATC11.3513513513513513No Hit
GGTACGTATGAAATTAGGAATATTAGTCGTGAACCA11.3513513513513513No Hit
GTTGTAATCCTTTTTCTTGGAACAAACAAACAGATT11.3513513513513513No Hit
CTGTAATTGTCATCATTTGGTAACGCGATCATATTC11.3513513513513513No Hit
ATCCTAAAGCATAACATTCTTTCTGTCCATCATGGC11.3513513513513513No Hit
ACCGTGACAATGGGGTGACTGCGAACATCTCATTTG11.3513513513513513No Hit
ATAAAGGGCTATAAAGTGAGTTTGCCAATCGAAAAA11.3513513513513513No Hit
CTTTGGAAGCGGCATAGTGGAAGTAAAAGTAGGCAA11.3513513513513513No Hit
TTCCATACCAGATTTGCTCCTGCGGCACCAGAAATT11.3513513513513513No Hit
ATTAATCTGATTGTAAAAATCCACGCAATGAACCGC11.3513513513513513No Hit
CAATAGTTCCGTTAGAAATTGACAAACCTTGACCAT11.3513513513513513No Hit
AGGTCACGGCGTCTGGCTACGAACCAGAAGCTTCCA11.3513513513513513No Hit
TTACAATACCGAGGATTTGGGTTCTCAAGGACCTCC11.3513513513513513No Hit
GCCCGGTGTACCAATATCATCTTGAGTAGGGACATA11.3513513513513513No Hit
TTACATGGGATGATAATATACAAATACCGTTCGCAA11.3513513513513513No Hit
ATGATAGACTGCACAATGAAAATGTAAACACAAATG11.3513513513513513No Hit
CAAAGTGTAACTGTAATATATCACCAATCTAACACG11.3513513513513513No Hit
TATCATAGAATACTCTTGTTGTTAATAATGGATTAT11.3513513513513513No Hit
TCCTTACACACGCCGCACGCAACCAGCACGCATCCA11.3513513513513513No Hit
ATGCTACATCATCTACACTTGAAGCACCCAGATTGG11.3513513513513513No Hit
ATGAAGAGATTTGTTGTTGCTTCAAACAAATACTTA11.3513513513513513No Hit
GAACTGTCGTATTTCTAGGTATCAAGGGTGAGAACG11.3513513513513513No Hit
ATTAGGTGCTGCTTACGGTACTGCTAAGACTGGTGT11.3513513513513513No Hit
ACTAAGCACTATTCATAAAGTTAAAATAAATCAATA11.3513513513513513No Hit
CTCTTAAATACATCTTAAAACAGCCTGCGATCGCAT11.3513513513513513No Hit
AGTAGGAAGTAAGCGTAGGGGGTGTAAGAAGGATGT11.3513513513513513No Hit
GAACGATACTTGTTCAGTGTTTACAAGACATAAGGT11.3513513513513513No Hit
ATATACGATGCCTCTTCACAGGATTAAGAGACAAAA11.3513513513513513No Hit
AAAGCAACGCTGGCACGTACTTCCGCCCTCAAGAAG11.3513513513513513No Hit
TTTTAAAAACAGAAATAAAAGGTTTAGAAAACCAAC11.3513513513513513No Hit
ATCTATCTCCGCCATAGAATCATCAATGGAAATACC11.3513513513513513No Hit
AGCCAATCTCTTGAAAGTACCTTCCTGTCTGCATTT11.3513513513513513No Hit
AAGATATAATAAAATATTTGAAAACATAAAATAATA11.3513513513513513No Hit
TTACTGAAGGGTGGTGAGCCTGATGAGTCTGGAGTT11.3513513513513513No Hit
TTACAATGTTTTATTCAATACAAAAGCAAATTCGAC11.3513513513513513No Hit
TCACAGTCCTCATACTATAACTGCATCCTATGAAGT11.3513513513513513No Hit
AATACACTCCAGGTGTAGCATCTGTACTTACATTGA11.3513513513513513No Hit
GGCCTAAGCAGCGGAGGCTGAATTTTAAAAGGCAGA11.3513513513513513No Hit
TTGCAATTGCAATTGCAATTGCAGCTGATTGCGAAA11.3513513513513513No Hit
GTATAATGAATGTTAAGGCCACTAAGAAAAAGATGA11.3513513513513513No Hit
GAAATAATACCAGGAGATCGTGTTCCTCCGAACGAG11.3513513513513513No Hit
GACCCCGGAGGATTTTGCGTCGCAAGGTGACTTAGT11.3513513513513513No Hit
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT11.3513513513513513No Hit
ACAAAGAAACCACAACTAAAACTCGATAAACGAAAA11.3513513513513513No Hit
ATTCAATGCGCTTATGGTGTATTTGGCAAGTTTTTG11.3513513513513513No Hit
CCATTAAGATAAACCGACTTGTACTTGAGTTTTTGG11.3513513513513513No Hit
TTCTAAGCCTTTGGGACCTGATGGAGATAAGTGGCT11.3513513513513513No Hit
GTATCCCCACAGGATTAATCCTGGGTGCTTCTGCGG11.3513513513513513No Hit
TATCAAATACAGGACTACAAATAGTACGCCCTCAAT11.3513513513513513No Hit
ACTATGAGTAGCAATTAAATAGGGTGTGGGGTACAA11.3513513513513513No Hit
TGCCAGAGCAACTGGACAACGACGACGAGAAGGAAG11.3513513513513513No Hit
CTCTACTACAAAGAACTACGGTAATGGTCAAGAAGA11.3513513513513513No Hit
CTACTAACTAGTATTTACGTTACTAGTATATTATCA11.3513513513513513No Hit
CATTAAAGATTAACCATCGAGATTAAAAGCCTAAAA11.3513513513513513No Hit
TCTCTGCTATCAAGCTCGTCGCGGCCCTGAGCCGTA11.3513513513513513No Hit
ATTATGTAAAACACCACCTAAGGTGCTTGTTCGTCA11.3513513513513513No Hit
CTCCAGATGTGGAAGCTAAGAAGTTCTTGGATTCAG11.3513513513513513No Hit
GATGGAAGCACTAATCGGTTCATCATCTATGTAAAG11.3513513513513513No Hit
GAAGGAGACAAAGGCAATAAGTGGTGCAAGTGCGAT11.3513513513513513No Hit
TAGAAAAGAGAGTAGGATTATATAAAATATAAAGAA11.3513513513513513No Hit
TATTGGAGGAACACCATTTGGAAACATGGATGCAAA11.3513513513513513No Hit
CACTAAATCAGAACAAATGCTCTCACATCATTATGC11.3513513513513513No Hit
CCTTTAGACTTACGTTTGCTACTCTCATGGTCTCAA11.3513513513513513No Hit
ACCCTGAGCTGATGCAATGTAAGTTATAATGTCAAA11.3513513513513513No Hit
GAGGAACAGTTCATTCGGATAATTGGTTTTTTCGGC11.3513513513513513No Hit
GACTAAGCCAGGGTGAAAAGACTAAACAAATTGAGC11.3513513513513513No Hit
CCAAAAATCTCACCGCATAAAATCACTTATCAAAAA11.3513513513513513No Hit
AATACCTAGAAACACACATTACCCGCCAAGCGATGC11.3513513513513513No Hit
GTCCCTAAGCAATACTTAAGTGATGAAGAGTTTTCT11.3513513513513513No Hit
GCTGGGGTGACAACAGGGGTGTTCAAACCACTCAAT11.3513513513513513No Hit
ATTCAGAATATTAGCAGACGCCTTTGAGCTGTATGA11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGATC50.029.99999813
TCCTCCG50.029.99999824
GTGTTCC50.029.99999820
GAAATAA50.029.9999981
GTTCCTC50.029.99999822
GATCGTG50.029.99999816
AAATAAT50.029.9999982
TGTTCCT50.029.99999821
GAACGAG50.029.99999830
TCGTGTT50.029.99999818
ATAATAC50.029.9999984
TTCCTCC50.029.99999823
CCTCCGA50.029.99999825
CGAACGA50.029.99999829
CGTGTTC50.029.99999819
TAATACC50.029.9999985
AATACCA50.029.9999986
ATCGTGT50.029.99999817
ATACCAG50.029.9999987
AATAATA50.029.9999983
Produced by FastQC (version 0.11.2)