FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_71.35100000039815.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_71.35100000039815.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77509
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCA1270.16385193977473583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCATC200.005124498629.9922587
ATCTCCG300.001218091524.9935510
CTCCGAG300.001218091524.9935512
CACGAGA300.001218091524.9935521
CGAGCCC300.001218091524.9935515
CCACGAG300.001218091524.9935520
GCCCACG350.00298568621.42304218
TCCGAGC350.00298568621.42304213
AGCCCAC350.00298568621.42304217
CCCACGA350.00298568621.42304219
CCGAGCC350.00298568621.42304214
GAGCCCA350.00298568621.42304216
TCTCCGA350.00298568621.42304211
CGAGACA350.00298568621.42304223
ACATCTC350.00298568621.4230428
ACGAGAC350.00298568621.42304222
ACACATC400.006418655618.769386
AAGAGGC400.00646675618.74516129
CATCTCC400.00646675618.7451619
CAAGAGG400.00646675618.74516128
Produced by FastQC (version 0.11.2)