FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_70.351000000397d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_70.351000000397d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586183
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA653.423734E-416.1595922
TATTATA952.621986E-514.215582
CATAAGA1201.1150896E-613.75488952
TATACTG1106.8763366E-613.6400485
TCTATAC902.6424218E-413.3369373
TATAAGG1056.7163775E-512.8617152
ATACTGC1201.6852255E-512.5033786
GAACCGC850.00260434212.356286
ACTGTGT850.002612690212.3510098
GTATAAA1406.2741747E-611.7899061
TATTATG900.00399245711.6708152
TATGACC900.00399500411.669824
ACCATCC1202.343782E-411.248248
GTGTAGG1355.6159206E-511.1150631
TGGGCTG950.005978630411.0556185
CTATACA1100.001427642910.9120394
AAGACCG1100.001427642910.9120395
ATATTAT1000.00874538410.5037341
TATTCCG1150.00206748910.4376035
CCATAAG1356.9216604E-410.0035561
Produced by FastQC (version 0.11.2)