FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_67.35100000039716.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_67.35100000039716.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861920
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG350.003006697621.42716217
TCGCGGG350.003006697621.42716216
GCGGGGG500.001110406817.99881718
AGGGGGG1101.2278178E-917.7281655
GGGTCCG550.002101992516.36256222
CTAAGGC755.615825E-516.0008053
TAGGGGG755.615825E-516.0008054
TCTAGGG809.7386546E-515.0016242
TAGGGCT706.032386E-415.0007534
ATAGGGG952.6323787E-514.2112413
GACAGGC650.006384665613.8460477
CATACAC1201.1206903E-613.7506913
ATACAGC902.6496107E-413.3340046
GTGAGTG850.00261149912.3528459
TATAGGG1354.1992334E-612.2235462
GGATATA1754.2162355E-812.0019961
AGACAGG1006.443361E-412.0006046
ACACATC1252.5705594E-512.0006036
AAGACTG2154.274625E-1011.8610615
ATACACA1901.0395524E-811.8427014
Produced by FastQC (version 0.11.2)