FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_65.351000000396ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_65.351000000396ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934979
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1152.220986E-916.95933
GTTACAC1352.894194E-713.3355163
TGGGGGG2451.8189894E-1212.2469025
ACCGCGT950.006002191511.051878
GTTCTAA2104.5514753E-810.7171481
AGGGGGG1551.9522593E-510.6469055
TACACTG3009.094947E-1210.50171855
TAGGGAG1150.002073027210.4364925
TAGGTCG1602.7715438E-510.3141885
TCTAGAC1952.1979486E-610.0016373
ACACCGT2209.896885E-79.5470176
CTAGTAC1757.4119045E-59.4301163
GCGTCAG1757.4119045E-59.4301164
GTCATAA2403.1970558E-79.3775041
TATACAC3951.8189894E-129.1154173
CTCTAGA1654.2719377E-49.0933371
GTATTAA3001.0526492E-89.0024041
TTATACT4550.08.9025564
CCTAATA2557.686449E-78.825432
TAAGACA2054.2949992E-58.7819254
Produced by FastQC (version 0.11.2)