FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_64.35100000039667.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_64.35100000039667.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233133
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG755.6117155E-516.0032224
GGACCGT650.00637787913.8489446
TAGGTAG1651.8580977E-812.7298375
GTCCTAA1302.7526257E-612.6948651
TGGGGGG1252.5686884E-512.0024175
AGGGGGG1501.0366584E-612.0024175
TAGGACT900.004001340411.6690174
GTATTAG2659.094947E-1211.3230351
CCTTATA2701.2732926E-1111.113352
ACATCGC1355.6527442E-511.1101958
AGTCTGC1501.3606959E-510.9990938
CTAGCCT1100.001430403810.9112894
CCCTAGA1253.415351E-410.80217551
GTCTAGA1408.1575825E-510.7164431
TCTATAC3800.010.6600423
ACTATGC3101.8189894E-1210.6442838
TTAGGTA2453.7089194E-910.410264
CTAAGGC1304.905746E-410.3867073
TATTAGG3051.2732926E-1110.329952
GACAGTG1602.7768381E-510.3116497
Produced by FastQC (version 0.11.2)