FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_63.35100000039624.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_63.35100000039624.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876890
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG805.1683965E-616.8778174
TGGGGGG2500.014.4024055
CGGGGGG750.001017082714.0023385
AGGGGGG2001.7334969E-912.0020045
CTAGGAC1406.3099924E-611.7873683
CTAGACT1202.3419813E-411.2518794
CTGAGCG1202.347198E-411.2491819
TTATACT5500.010.91091254
AGGGCTG1551.9521023E-510.6469395
CTAGGAT1451.1656531E-410.3462793
TAGACGG1756.602575E-610.2874335
TCTAGAC2053.7674E-710.2453393
CTATGCA3253.6379788E-1210.1531089
GTATTAG3550.010.143621
GTGCGCG1200.002957139.9992729
GCTGCGG1851.2798388E-59.73057728
TACACTG3551.8189894E-129.7199335
ACTACGC1705.3978343E-59.7072453
ACCACGC1409.7218796E-49.6421558
GTCTAGG2651.3440513E-89.6252741
Produced by FastQC (version 0.11.2)